MYD88 P.L265P MUTATION ANALYSIS

MUTATION TESTED: MYD88 p. L265P

INDICATIONS FOR ORDERING: Diagnostic in cases of lymphoplasmacytic lymphoma, Waldenstroms Macroglobulinemia. It is also found in 70% cases of Primary testicular diffuse large B cell ymphomas and in a few cases of Primary central nervous system lymphoma.

METHOD USED: Allele-specific oligonucleotide polymerase chain reaction for the detection of MYD88 p.L265P mutation analysis

SPECIMENS – Type, storage and transport

• Type: Non-diluted peripheral blood sample/bone marrow aspirate in an EDTA (lavender) tube. PLEASE AVOID HEPARINATED BLOOD

Bone Marrow: Min. 3mL

Whole Blood: 3 mL

• Transport: Specimens must be received within 48 hours of collection
• Storage: Whole Blood or Bone Marrow. Separate specimens must be submitted when multiple tests are ordered.
• Extracted DNA: Separate specimens must be submitted when multiple tests are ordered.
• Unacceptable samples: Specimens collected in anticoagulants other than EDTA, haemolysed/clotted samples

TEST DESCRIPTION: This is a DNA-based PCR assay. The purified DNA sample is subjected to PCR assay using specific primer sets for wildtype and mutant MYD88 p.L265P mutation.

In case of no amplification signal for a wild type in biological negative control, a repeat sample is requested in view of any cross contamination

ANALYTICAL SENSITIVITY: This is a qualitative test, hence yields a positive or a negative result only.

TURNAROUND TIME: 5 (five) working days from receipt of sample

QUALITY ASSURANCE & COMPETENCY TESTING: Regular internal quality checks and intra-lab comparisons are performed to ensure accurate and precise results. Material Safety Data Sheets (MSDS) may be ordered upon request. Highly trained personnel performing this test undergo regular competency assessments and attend regular conferences and educational programs in order to stay upbeat with the newer advances in the biology and diagnostics of the disease in question.

ACCREDITATION: NOT IN SCOPE OF NABL

NOTE: This is a lab-developed tests and regular quality checks and controls are run to ensure correctness of the report.