VIABLE CELLS

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Viable Cells

Dissociated Tumor Cells (DTC’s):

Dissociated tumor cells refer to individual or small clusters of cancer cells that have been separated from a solid tumor mass by shearing the tumor using mechanical or enzymatic methods. Isolated cells can be analyzed for genetic mutations and studied for growth patterns and drug sensitivity. Isolated cells can be studied for their heterogeneity and differential gene expressions. Isolation method can greatly affect final cell count and viability of cells. We have standardized our methods to cryostore high counts pre freeze (~90%) and post thaw (~70-80%).

Availability: DTC’s are prepared on a prospective basis with around 25 DTC’s in the banked inventory for immediate sharing .

Peripheral Blood Mononuclear cells (PBMC’s):

Peripheral Blood Mononuclear Cells (PBMCs) are cells bearing a single round nucleus and are found in peripheral blood. These are primarily composed of lymphocytes and monocytes. PBMCs isolation is done from peripheral blood using density gradient centrifugation techniques. One method involves layering blood over a density gradient medium, such as Ficoll-Paque, and centrifuging it to separate PBMCs from other blood components. PBMC’s are extensively researched in immunooncology therapeutics. Cryopreservation of PBMC’s is done to maintain their viability and functionality by using cryoprotectants like dimethyl sulfoxide (DMSO) and then isolated cells in aliquots are stored at ultra-low temperatures. We have standardized our methods to cryostore high counts pre freeze (~90%) and post thaw (~60-75%).

Availability: PBMC’s are prepared on a prospective basis with around 20 DTC’s in the banked inventory for immediate sharing .