BRAF MUTATION ANALYSIS BY REAL TIME QUALITATIVE PCR

MUTATION TESTED: PCR-based assay to detect mutations in BRAF gene INDICATIONS FOR ORDERING: Malignant melanoma, Hairy cell leukemia, colorectal and lung malignancies METHOD USED: BRAF Analysis Kit by Entrogen. We also perform genomic IHC for BRAF V600E using VE1 clone using Ventana platform SPECIMENS – Type, storage and transport

• Type: Non-diluted peripheral blood sample/bone marrow aspirate in an EDTA (lavender) tube. Formalin fixed paraffin embedded tissue sections (Tissue block).PLEASE AVOID HEPARINATED BLOOD

Bone Marrow: Min. 3mL Whole Blood: 3 mL

• Transport: Specimens must be received within 24 hours of collection
• Storage: Whole Blood or Bone Marrow. Separate specimens must be submitted when multiple tests are ordered.
• Extracted DNA: Separate specimens must be submitted when multiple tests are ordered.
• Unacceptable samples: Specimens collected in anticoagulants other than EDTA, haemolysed/clotted samples

TEST DESCRIPTION: This is a DNA based real time qualitative PCR based assay which detects mutations in exon 15 of the BRAF genes which are commonly implicated in the malignancies enlisted above. Alternatively, immunohistochemistry can be performed for BRAF V600E mutation on tissue blocks, using the Ventana system TURNAROUND TIME: 5 (five) working days from receipt of sample QUALITY ASSURANCE & COMPETENCY TESTING: Regular internal quality checks and intra-lab comparisons are performed to ensure accurate and precise results. Material Safety Data Sheets (MSDS) may be ordered upon request. Highly trained personnel performing this test undergo regular competency assessments and attend regular conferences and educational programs in order to stay upbeat with the newer advances in the biology and diagnostics of the disease in question. ACCREDITATION: NABL