MICROSATELLITE INSTABILITY TESTING

MICROSATELLITE INSTABILITY TESTING BY FRAGMENT LENGTH ANALYSIS

MUTATION TESTED: PCR-based assay to detect microsatellite instability (MSI) in human cells comparing allelic profiles of microsatellite markers.

INDICATIONS FOR ORDERING: Biomarker for immunotherapy

METHOD USED: MSI Analysis Kit 1.2, Promega. Alternatively, MMR protein can be valuated using immunohistochemistry.

SPECIMENS – Type, storage and transport

• Type: Non-diluted peripheral blood sample/bone marrow aspirate in an EDTA (lavender) tube. Formalin fixed paraffin embedded tissue sections (Tissue block).PLEASE AVOID HEPARINATED BLOOD

Bone Marrow: Min. 3mL

Whole Blood: 3 mL

• Transport: Specimens must be received within 48 hours of collection
• Storage: Whole Blood or Bone Marrow. Separate specimens must be submitted when multiple tests are ordered.
• Extracted DNA: Separate specimens must be submitted when multiple tests are ordered.
• Unacceptable samples: Specimens collected in anticoagulants other than EDTA, haemolysed/clotted samples

TEST DESCRIPTION:. The National Cancer Institute Workshop agreed on five microsatellite markers necessary to determine MSI (5) that include two mononucleotide – BAT25/26 and three dinucleotide markers – D2S123, D5S346, and D17S250. Interpretation of the profiles requires a comparison with normal DNA from each patient. On the basis of the MSI status, CRCs can be classified into three groups: MSI-H, if two or more of the five microsatellite markers show instability; MSI-L (low-frequency MSI), if only one of five markers shows instability; and microsatellite stable (MSS) if none of the markers show instability.

TURNAROUND TIME: 5 (five) working days from receipt of sample

QUALITY ASSURANCE & COMPETENCY TESTING: Regular internal quality checks and intra-lab comparisons are performed to ensure accurate and precise results. Material Safety Data Sheets (MSDS) may be ordered upon request. Highly trained personnel performing this test undergo regular competency assessments and attend regular conferences and educational programs in order to stay upbeat with the newer advances in the biology and diagnostics of the disease in question.

ACCREDITATION: NABL