NRAS & KRAS MUTATION TESTING BY REAL TIME QUANTITATIVE PCR

MUTATION TESTED: PCR-based assay to detect mutations in NRAS and KRAS genes

INDICATIONS FOR ORDERING: Gastrointestinal and lung malignancies

METHOD USED: NRAS Mutation Analysis Kit, and KRAS Mutation Analysis Kit (EntroGen)

SPECIMENS – Type, storage and transport
• Type: Formalin fixed paraffin embedded tissue sections (Tissue block). Please provide a hematoxylin and eosin stained slide of the corresponding block, if available

• Transport: Specimens must be received within 48 hours of collection
• Storage: Separate specimens must be submitted when multiple tests are ordered.
• Extracted DNA: Separate specimens must be submitted when multiple tests are ordered.
• Unacceptable samples: Depleted tissue blocks, Non-representative tissue blocks

TEST DESCRIPTION: This is a DNA based real-time PCR assay which investigates mutations in the KRAS and NRAS genes. The mutations detected by these are enlisted in the table below. Please note only the exon and codon can be commented here. The specific protein change cannot be ascertained.

TURNAROUND TIME: 5 (five) working days from receipt of sample

QUALITY ASSURANCE & COMPETENCY TESTING: Regular internal quality checks and intra-lab comparisons are performed to ensure accurate and precise results. Material Safety Data Sheets (MSDS) may be ordered upon request. Highly trained personnel performing this test undergo regular competency assessments and attend regular conferences and educational programs in order to stay upbeat with the newer advances in the biology and diagnostics of the disease in question.

ACCREDITATION : NABL

Mutations tested